![]() Single-cell RNA-seq data reduced to two dimensions with PCA and UMAP. Will create animated visualizations to see how the algorithm works and developĪn intuitive understanding. Problem, the Harmony algorithm iteratively clusters and adjusts highĭimensional datasets to integrate them on a single manifold. When we know that all of the datasets have similar cell types. ![]() That each dataset is separated from the others in low dimensional space – even WhenĪnalyzing multiple single-cell RNA-seq datasets, we often encounter the problem ![]() Harmony is a an algorithm for aligning multiple high-dimensional datasets,ĭescribed by Ilya Korsunsky et al.
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